If one mentions sewage, you will no

doubt begin to think of a whole host of viruses (including SARS-CoV-2) and microbes (perhaps some as yet unknown) that inhabit this unique ecosystem. As the world population increases and more waste is produced, there needs to be more research into wastewater and sewage if we are to maintain a healthy balance of population to waste.

 

Obtaining and studying nucleic acids from water and waste samples for metagenomic studies has always been challenging due to the presence of PCR inhibitors, heavy metals and other contaminants. 

 

The MasterPure™ Complete DNA and RNA Purification Kit is recognised as a very versatile and adaptable kit for DNA/RNA purification. This is because, unlike silica-based kits, the  Masterpure Complete™ range of kits are all liquid based. Thus there are no sample volume limitations, clogged filters, and most importantly there are minimal losses of nucleic acids resulting in smooth workflows and robust yields. The purified RNA and DNA is inhibitor-free and suitable for any any downstream application.

 

If you plan to analyse SARS-CoV-2 variants or other nasties an application note for obtaining RNA and DNA from water can be found here.

 

For more info please contact support@cambio.co.uk.