How Can Transposons Accelerate Your Genomic Research?
Have you ever generated libraries of mutants or gene knockouts, but struggled to get complete representation in your library? Do you need techniques to help easily identify essential genes or regulatory elements? Perhaps you have struggled with de novo sequencing of large genomes, or rescuing plasmid DNA from environmental samples in metagenomics screens? In this webinar, we will talk about some of the challenges inherent in these applications, and how transposons, or “jumping genes”, can help. We will introduce EZ-Tn5 transposon mutagenesis as a well-known, easy-to-use system that employs an engineered, hyperactive Tn5 Transposase enzyme that is 100 times more active than the native Tn5 transposase. We will discuss how transposons are used by many researchers for metagenomics, bacterial strain development and gene knockout mutagenesis in vitro and in vivo. We will also present techniques for introducing mutations in bacterial genomes using simple electroporation, “rescuing” non-coliform plasmid DNA from environmental organisms, and creating custom transposons using PCR for novel applications. In addition, we will address customer questions and provide troubleshooting tips.
Key Learning Objectives
1 The Irwin Centre, Scotland Road, Dry Drayton, Cambridge, CB23 8AR
tel: 01954 210200
fax: 01954 210300
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